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. 2014 Sep 3;11:149. doi: 10.1186/s12974-014-0149-8

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© Jose et al.; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Mesenchymal stem cells inhibits BV2 microglia proliferation independent of nitric oxide. (a) BV2 cells and Mesenchymal stem cells (MSC) were seeded at a 1:0.2 ratio in a 96-well plate with 1 μg/ml lipopolysaccharide (LPS) and 1000 μM N-nitro-L-arginine methyl ester (L-NAME) for 48 hours, and BV2 proliferation was determined with a tritiated thymidine (³H-TdR) incorporation assay. Values are expressed as mean ± SD of percentage BV2 proliferation from three independent experiments. (b) NO₂ ^- concentration in culture supernatant was determined using the Griess assay. BV2 cells and MSC were cultured in 12-well plates at a 0.2 ratio (BV2: MSC) and stimulated with 1 μg/ml LPS, and NO₂ ^- was assayed at 48 hours. Values are expressed as mean ± standard deviation of NO₂ ^- in μM from three independent experiments. *P < 0.01, versus BV2 cells; #P < 0.01, versus BV2 + LPS.