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. 2014 Sep 3;11:149. doi: 10.1186/s12974-014-0149-8

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© Jose et al.; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Mesenchymal stem cells inhibit microglia proliferation through contact-dependent cell cycle modulation. BV2 and Mesenchymal stem cells (MSC) were cultured at a 1:0.2 ratio and stimulated with 1 μg/ml lipopolysaccharide (LPS) after overnight incubation. Cells were harvested 48 hours post-LPS stimulation and cell cycle was analysed by propidium iodide staining. Separation of BV2 and MSC populations on flow cytometry was enabled by CD45 staining (see Additional file 3: Figure S3). Histograms show distribution of (a) BV2 cells and (b) MSC from direct co-culture across G0/G1, S and G2/M phases of cell cycle at 48 hours after LPS stimulation and/or co-culture. Values are expressed as mean ± standard deviation of percentage of cells from three independent experiments. (a) *P < 0.01, versus BV2 cells; #P < 0.01, versus BV2 + LPS. (b) *P < 0.001, versus MSC; #P < 0.001, versus MSC + LPS.