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. 2014 Sep 2;107(5):1129–1135. doi: 10.1016/j.bpj.2014.07.016

Figure 2.

Figure 2

Typical decrease of the FRAPP fringes contrast as a function of time (here for SERCA1a in L3). From the fit of this curve (measured at an interfringe distance i = 19.4 μm) we obtain a characteristic decay time τ = 5.2 s, which corresponds to a D value of 1.7 ± 0.1 μm/s2. In the inset, we present the variation of 1/τ as a function of (i/2π)2. The linear behavior indicates the Brownian motion of the protein embedded in the bilayers of L3 phase and the slope of the linear fit leads to the self-diffusion coefficient of the protein.