Figure 1.

Interaction of GlialCAM with CLC-1. (A–D) Cellular distribution of CLC-1 in HeLa cells. CLC-1 alone is located uniformly in the plasma membrane and intracellularly (A, B) whereas GlialCAM changes CLC-1 localization when both proteins are co-transfected to regions of direct contact (C, arrow) or to cell-cell contact processes (D, arrow). (E–H) GlialCAM activates the slow gate of CLC-1 (inset: protocol to determine the open probability of the slow gate; see Methods). (E, F) typical currents from a patch from oocytes expressing CLC-1 alone (E) or CLC-1 with GlialCAM (F). (G) Initial (I_o) tail currents from the patches shown were described with a Boltzmann function (plus offset) and the open probabilities of the example patches are plotted against voltage. The relative offset of the slow gate (H) is significantly larger with GlialCAM (^∗∗p < 0.01, Student’s t-test, values are mean ±SE). Voltages of half-maximal activation were not significantly different (data not shown). To see this figure in color, go online.