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. 2014 Sep 2;107(5):1105–1116. doi: 10.1016/j.bpj.2014.07.040

Figure 4.

Figure 4

GlialCAM activates the slow gate of CLC-0. (A) Pulse protocol used to assay the slow gate. (B, C) Typical current traces obtained with this protocol for CLC-0 (B) and CLC-0/GlialCAM (C). (D) Example tail currents from the current traces shown in (B) and (C) plotted as a function of the prepulse voltage and fitted with a Boltzmann function with offset (lines). (E) Maximal current at 40 mV obtained from the Boltzmann analysis (F) Relative offset obtained from the Boltzmann analysis (∗∗∗p < 0.001, Student’s t-test). (G) GlialCAM-induced clustering of CLC-0-GFP in HEK293 cells. (H) Dose-response of increasing concentration of GlialCAM on relative offset of the slow gate. Amount of CLC-0 RNA was 0.25 ng/oocyte. Data were fitted by a “Boltzmann equation” resulting in a half maximal amount of 0.25 ng/oocyte GlialCAM RNA (qualitatively similar results were seen in a total of three batches of oocytes). To see this figure in color, go online.