Figure 5.

Temperature sensitivity of the deactivation kinetics of the slow gate. (A) Current decay at resting conditions, monitored by brief pulses every 2 s to 40 mV, after a long hyperpolarizing pulse (T = 33°C for this example). (B, C) Current deactivation was described by a single exponential function yielding the time constant (B) and the percentage of deactivation (C) as a function of temperature. Due to the very slow deactivation of CLC-0/GlialCAM at lower temperatures, it was not possible to obtain a reliable value for the time constant (n = 4 to 7 for each temperature from a total of 5 to 10 different oocyte batches; error bars represent SE).