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. 2014 Sep 2;107(5):1105–1116. doi: 10.1016/j.bpj.2014.07.040

Figure 8.

Figure 8

CLC-2 double gating mutant E211V/H816A expressed in HEK cells and oocytes. (A, B) Typical current traces obtained by a voltage pulse to 60/–120/60 mV in oocytes (A) and HEK293 cells (B) for the double mutant without (top) or with (bottom) GlialCAM. (C) Fluorescence of HEK cells expressing the E211V/H816A mutant C-terminally fused to GFP without (top) or with (bottom) GlialCAM. (D) Imax at 60 mV was slightly increased for E211VH816A when co-expressing GlialCAM in oocytes (∗p < 0.05, Bonferroni’s test). Note, that in patch clamp experiments of HEK cells GlialCAM does not increase the currents of the double mutant. (E) In contrast to CLC-2 co-expressing the double gating mutant with GlialCAM does not alter its current characteristics as assayed by the ratio of steady state currents and Imax at 60 mV (n ≥ 6 cells from two different transfections/injections; error represents SEM). To see this figure in color, go online.