Table 1.
Effect of GlialCAM on biophysical properties of different CLC channels and transporters
| Clustering strength∗ | Increase of Imax at positive V (in oocytes) | Activation of slow gate† | Slowed kinetics of deactivation at positive voltages‡ | |
|---|---|---|---|---|
| CLC-2 | +++ | 15-fold | +++ | +++ |
| CLC-2E221V/H816A | +++ | No | - | n.a. |
| CLC-1 | +++ | No | + | n.a. |
| CLC-0 | ++ | 3.9-fold | +++ | +++ |
| CLC-Ka | + | No | n.a. | n.a. |
| CLC-Ka+Barttin | + | No | n.a. | + |
| Barttin-2A-CLC-K1 | ++ | No | n.a. | + |
| CLC-5 | - | No | n.a. | n.a. |
∗
The strength of interaction is qualitatively indicated by the number of + signs reflecting the number of clusters found per number of transfected dishes: CLC-2: 9/3; CLC-2E221V/H816A: 21/7; CLC-1: 28/8; CLC-0: 19/8; CLC-Ka: 13/13; CLC-Ka+Barttin: 9/13; Barttin-2A-CLC-K1: 9/4; CLC-5: 0/3. †the number of + signs qualitatively indicates that the slow gate is strongly activated in CLC-0 and CLC-2 but only slightly in CLC-1; ‡the number of + signs qualitatively indicates that the deactivation kinetics is strongly affected in CLC-2 and CLC-0 and less in CLC-Ka. n.a.: not applicable.