FIG. 7.
Cytokine production in DC and T-cell cocultures. Day 5 BM-derived DC generated from BALB/c mice were harvested, purified for CD11c-positive cells, and treated with probiotic or bacteria (105 organisms/ml). Treated DC-purified T-cell cocultures were set up with 0.5 × 106 DC and 1.5 × 106 T cells in 24-well plates. Supernatants were harvested at day 3 and frozen at −20°C for cytokine analysis by ELISA. D alone represents DC precultured in PBS; similar results were obtained with those precultured in bacteria or probiotic. D(V5), DC pretreated with VSL#3 at 105 organisms/ml; D(LC5), pretreated with L. casei; D(EC5), those pretreated with E. coli. Values represent means ± 1 standard deviation of the results for duplicate wells from two experiments performed. (A) IL-10 production; (B) IFN-γ production in cocultures.