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. 2004 Jun;72(6):3418–3428. doi: 10.1128/IAI.72.6.3418-3428.2004

FIG. 5.

FIG. 5.

DltA binding to immobilized CHO. Zwittergent 3,14-soluble total H. ducreyi proteins from ∼109 CFU were mixed with and allowed to bind to acrylamide beads containing the indicated covalently bound CHO or glycoprotein. After the unbound components were removed, the pellet was solubilized in Laemmli sample buffer and then subjected to SDS-PAGE under nonreducing conditions and Western blotting with anti-rDltA (panel 1) or DIG-FN (panel 2). Lanes: 35000HP-WC(+), Zwittergent 3,14-solubilized proteins from whole H. ducreyi cells (∼107 CFU/lane) used as positive control; GALNAC, N-acetylgalactosamine; GLCNAC, N-acetylglucosamine; BSM, bovine submaxillary mucin.