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Cell surface iodination of whole H. ducreyi cells. Whole cells of H. ducreyi (ca. 5 × 108 CFU) were surface labeled with 125I and subjected to SDS-PAGE in reducing conditions. The gel was stained with Coomassie blue stain, destained, and dried. The left panel of the figure shows the Coomassie blue-stained gel, and the right panel shows the autoradiograph.
