Fig. 1.

Immunolabeling of tissues from S. pistillata embedded in paraffin. Tissue sections contain calcifying and noncalcifying cells as indicated by the headings. IHC of seven distinct SOM proteins reveals labeling at distinct intracellular localizations for each protein (brown), counterstained with hematoxylin (blue). (Insets) Specific localization of individual antibody labeling (magnification of 100×). BBWG, basal body wall gastrodermis; Ca, calicodermis; Ep, epidermis; GC, gastrovascular canal; N, nematocyte; SBWG, surface body wall gastrodermis; Sk, skeleton side; SW, seawater side; Z, zooxanthellae. (Scale bars: 20 μm.)