. 2014 Aug 18;111(35):E3650–E3658. doi: 10.1073/pnas.1402617111

Table 1.

Growth characteristics of Dokdonia sp. MED134 at different light conditions

Seawater culture enrichment	Treatment	Specific growth rate (µ_max, h⁻¹)	Cell yield (10⁶/mL)	Cell volume (μm³)^*	Cell production (10⁵ mL⁻¹⋅h⁻¹)^†	Carbon production (fgC⋅cell⁻¹⋅h⁻¹)^‡	Bicarbonate incorporation (fgC⋅cell⁻¹⋅h⁻¹)	Bicarbonate incorporation:carbon production (%)^§
YEP	Light	0.059 ± 0.007	25.9 ± 1.4	0.044 ± 0.005	7.59 ± 0.84	1.92 ± 0.39	0.47 ± 0.06	24.5 ± 5.9
YEP	Dark	0.048 ± 0.011	19.5 ± 1.1	0.036 ± 0.004	4.43 ± 1.41	1.84 ± 0.28	0.10 ± 0.01	5.4 ± 1.0
Ala	Light	0.074 ± 0.001	12.3 ± 0.6	0.035 ± 0.004	2.42 ± 0.46	0.45 ± 0.09	0.14 ± 0.02	31.1 ± 7.9
Ala	Dark	0.031 ± 0.004	2.9 ± 0.2	0.029 ± 0.003	0.69 ± 0.09	0.79 ± 0.12	0.11 ± 0.01	13.9 ± 2.0

Cultures were incubated in the light and in darkness in artificial seawater enriched with either a mixed (YEP) or a single (Ala) dissolved organic carbon source. Also shown is comparison of bicarbonate incorporation in relation to cell carbon production in the different treatments. Values represent mean ± SD for three biological replicates, except for bicarbonate incorporation where values represent mean ± SD for four subsamples of duplicate biological replicates.

Mean cell volume during growth at the time of bicarbonate incorporation measurements (Fig. 1).

^{^†}

Calculated from 33.75 to 45.25 h (YEP) and 45.75 to 62.75 h (Ala) (Fig. 1).

^{^‡}

Per cell carbon production calculated from abundance at the time of bicarbonate incorporation measurements, cell production, and carbon per cell. Carbon per cell values were calculated based on measured cell volumes and the experimentally determined strain-specific carbon content for Dokdonia sp. MED134 cells growing in YEP; values for cells growing in the light or in the dark were 515 ± 103 (n = 3) or 778 ± 115 µgC^⋅µm⁻³ (n = 3), respectively (Student t test, P = 0.015). The YEP light and dark carbon content values were applied also to the cells growing in the Ala light and dark cultures, respectively. Strain-specific carbon content errors have been used in error propagation calculations for each biological replicate to determine the SDs for carbon production. Resulting carbon per cell values ranged from 28.2 fgC⋅cell⁻¹ in YEP to 18.2 fgC⋅cell⁻¹ in Ala.

^{^§}

SDs represent the relative cumulative error obtained by error propagation calculations from carbon production and bicarbonate incorporation.