Table 1.

PARP-1 affinities measured by HI-FI FRET

	N-parp			PARP-1			AM–PARP-1
Binding substrate	Approximate Kd, nM	R2	No. rep.	Approximate Kd, nM	R2	No. rep.	Approximate Kd, nM	R2	No. rep.
30Nick	27.8 ± 5.6	0.95	5	23.4 ± 4.8*	0.98	2	33.2 ± 23.5*	0.94	5
Nuc207	48.8 ± 21.2	0.97	2	1.0 ± 0.2	0.90	2	13.2 ± 2	0.96	5
LE-Tri	20.3 ± 2.6	0.97	1	12.7 ± 6.4	0.95	2	10 ± 2	0.95	4
NLE-Tri	22.8 ± 4.8	0.95	1	4.8 ± 2.1	0.98	3	101 ± 23	0.94	3
H2A-H2B	>500	0.94	1	>500	0.93	4	2.3 ± 0.8	0.94	4
H3-H4	>500	0.98	1	>500	0.99	3	16 ± 3	0.96	3

^*Amount used is 250 mM NaCl instead of 200 mM NaCl.

Each data point is an average of two to five individual experiments, each performed in duplicate; experiments with N-parp were performed once, in duplicate. Values listed in italics are published (7), but they are shown here for comparison. No. rep., number of independent replicates for each value.