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. 2014 Aug 19;111(35):12716–12721. doi: 10.1073/pnas.1410555111

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Fig. 2. — (A) A germ-line chimeric male KOC founder (#4312) artificially inseminated a female WL. (B) The hatched chicks produced from the founder through test-cross and T7E1 assay of the offspring. Feather color of the donor WL (I/I)-derived chick was white, whereas the hybrid (I/i) chick produced from the recipient founder KOC had black spots. OV mutant chicks were identified by T7E1 assay. (C) Targeted DNA sequences in the OV gene were analyzed in the OV mutant donor PGC-derived offspring. A dash in the DNA sequences denotes deleted nucleotides, and bold ATG indicates the translational initiation codon in the second exon of the OV gene. (D) Detection of the OV TALEN 2 construct in mutant offspring by genomic PCR. OV TALEN 2 expression vector concentration was used as a series of 10-fold dilutions from 100 pg to 10 fg. Wild-type chicken genomic DNA was used as a negative control.