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Indirect methods for measuring hapten density of protein conjugates. Modified Ellman’s test was performed by treating maleimide-BSA intermediate with excess MESNA and subsequently back titrating the unreacted MESNA with Ellman’s reagent (a). TNBS assay was executed by reacting MorHap-BSA conjugates with TNBS in basic buffer (b). Both chemical assays measured hapten equivalences
