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. 2004 Jun;72(6):3451–3460. doi: 10.1128/IAI.72.6.3451-3460.2004

FIG. 5.

FIG. 5.

Apparent dissociation half-lives of anti-MCPS Abs. The apparent dissociation half-lives were calculated by exponential decay modeling as described in Materials and Methods. (A) Averages of four determinations after injection of Abs C2/1076.10, 2016.3, and C2/655.7 at a concentration of 3.125 or 6.25 μg/ml are shown for OAc− and OAc+ surfaces. The error bars represent the standard deviations, and the numbers in parentheses are the numerical averages. For Ab 2055.5, the bars and averages reflect two determinations and the error bars reflect the range. (B) Averages of four determinations using polyclonal sera after injection of 1/20 or 1/40 dilutions; determinations are shown for OAc− and OAc+ surfaces. The error bars represent the standard deviations, and the numbers in parentheses are the numerical averages. (C) Examples of SPR sensorgrams of Vac2 immune sera binding to OAc−-BSA conjugate (242.8 RU immobilized) at concentrations of 1/10, 1/20, and 1/40.