TABLE 3.
Relative avidities of total IgM and IgG from murine sera by FELISA
| Immunization | Relative avidity for MCPS Ag on platea
|
|||
|---|---|---|---|---|
| OAc+
|
OAc−
|
|||
| IgM | IgG | IgM | IgG | |
| PBS | 0.05 | >1 | 0.03 | >1 |
| OAc+ bacteria | 0.005 | 0.4 | 0.004 | 0.35 |
| OAc− bacteria | 0.01 | 0.75 | 0.004 | 0.65 |
| Vac1 | 0.05 | 0.0002 | 0.04 | 0.001 |
| Vac2 | 0.03 | 0.0002 | 0.09 | 0.001 |
| Vac3 | 0.02 | 0.0006 | 0.03 | 0.0002 |
a
Values are reciprocal dilutions for 50% binding defined as an optical density of 2 on a scale of 0 to 4 for each serum from mice immunized with different bacterial strains or conjugate vaccines as indicated. The data are from similar results in two experiments. PBS was used as a control.