TABLE 1.
Primers used in this study
| Target | Primera | Sequence (5′-3′) | Reference |
|---|---|---|---|
| spt1R (fasA) | spt1R-L | TACCATGTAATCAGCTTGAA | This study |
| spt1R-R | AACAAACTTTGTGAGGAGT | This study | |
| fasA-R1* | GGGATTGATTGCTCGATAAA | This study | |
| fasA-L1 | AGCACAAAAACCAATCGTGT | This study | |
| spt2R (covR) | covR-L | TAGTGAGAGAAATCTCATCG | 5 |
| covR-R | TATGAAGTCATTGTTGAGGT | 5 | |
| covR-R1* | AGGCAATCAGTGTAAAGGCA | This study | |
| covR-L1 | AATCCTTTTGCTAGCTTGCA | This study | |
| spt4R | spt4R-L | AAGGCCATTAATTTACCTTC | This study |
| spt4R-R | CGGTCAAGAGGCAATTGATA | This study | |
| spt4R-R1* | GCATCTTGTGATGTTACCAT | This study | |
| spt4R-L1 | CGGTTAATTTCTTGTTGACT | This study | |
| spt5R | spt5R-L1 | AAGAAGGAGCTGTTTTAATC | This study |
| spt5R-R1 | CCTGATTAATCCGATATTGA | This study | |
| spt5R-R2* | GCCATTTGCGTTTGAGTTTT | This study | |
| spt5R-L2 | TTCGGGAATTGATGCTCATC | This study | |
| spt6R | spt6R-L | ATTCTGAAGCTTATGAAGAC | This study |
| spt6R-R | TGACGTGACAGTAATTCTAA | This study | |
| spt6R-L2 | AGAGATCTTGAGTGATAGCA | This study | |
| spt6R-R2* | TAAACATCCCATTTATAACC | This study | |
| spt7R | spt7R-L1 | ACAAGCGGAAGCTAATTCTT | This study |
| spt7R-R1 | ATCCAGCGACTCTATGAAAG | This study | |
| spt7R-R2 | TCTCAGTGACAACACCTTAT | This study | |
| spt7R-L2* | GTTTATGACAATTTAGGGTC | This study | |
| spt8R | spt8R-L1 | ATCACTATCGAATCCCCATA | This study |
| spt8R-R1 | TGAAGGTTTATACGAAGCAG | This study | |
| spt8R-L2 | AAACTACTGTCAACTGACGA | This study | |
| spt8R-R2* | GGCTATTAGCCTCTTAAATA | This study | |
| spt9R | spt9R-L | TTTTAATCAAGACGCTAAGG | This study |
| spt9R-R | GTTGATTTTAGCCAGTTCAA | This study | |
| spt9R-R1* | ATATGGAAGCTAAGGGCTAT | This study | |
| spt9R-L1 | TACGAGAACTCGTCTGGTAA | This study | |
| spt10R | spt10R-L | AGTGGATGTCATGATTTCAG | This study |
| spt10R-R | CGCTTTTGTAGATCATAGGT | This study | |
| spt10R-R1* | TTGGTAGCCAGTCACTGTCT | This study | |
| spt10R-L1 | TCAAGGCTTTAGATGAGACG | This study | |
| spt11R | spt11R-L | TCCGCATGGGACTCAAGAGT | This study |
| spt11R-R | GTCAGGGTGTTGATCATGCG | This study | |
| spt11R-R1* | AGGCATATAACCCGTATCTT | This study | |
| spt11R-L1 | TTGAAGATGATTGATGATGG | This study | |
| spt12R | spt12R-L | GCAAAGAGTATCCAACTGTT | This study |
| spt12R-R | TTCCTGGTCAGTTAAAGATT | This study | |
| spt12R-R1* | TAGCAACGACACAAGTAAAA | This study | |
| spt12R-L1 | TTCAAGAAACACTAGCAGCT | This study | |
| spt13R (irr) | irr-L | GGTGACGTTTTGCTAAATAA | 5 |
| irr-R | AAAGCGAATAACTATGATCC | 5 | |
| irr-R1* | TGTCTTTGGACTATTACCAG | This study | |
| irr-L1 | CTTCTTTGTCTTTGACTTTG | This study | |
| M13 | 1201 | AACAGCTATGACCATGATTACG | Clontech |
| 1211 | GTAAAACGACGGCCAGT | Clontech | |
| pWV01 ori | pLZ12ori-L | TTATATCCTGACTCAATTCC | This study |
| pLZ12ori-R | CTCAAACCATAATCTAAAGG | This study | |
| emm | OM6-35 | AACAGCAAATTAGCTGCTC | 15 |
| OM6-16 | GTTTCCTTCATTGGTGCT | 15 | |
| 23S rRNA | rRNA_23S-L | GGAAGGTAAGCCAAAGAGAG | 20 |
| rRNA_23S-R | TCCTAGTTTCTGTGCAACC | 20 |
a
Boldface indicates wild-type sptR gene pair. An asterisk indicates integrant junction primer.