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. 2004 Jun;72(6):3138–3146. doi: 10.1128/IAI.72.6.3138-3146.2004

FIG. 5.

FIG. 5.

Effects of proteinase K treatment of different strains of B. burgdorferi. (A) At increasing concentrations of proteinase K, there was increased digestion of OspA, while the amount of FlaB remained unchanged. (B) Total cell extracts from B. burgdorferi strains B31-UM, B31, B314, MC-1, and N40 (lanes 1, 3, 5, 7, and 9; digested with 5 mg of proteinase K/ml, lanes 2, 4, 6, 8, and 10) were separated by electrophoresis on SDS-12% polyacrylamide gels. Proteins were detected by immunoblotting with antibodies.