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Validation of the SILAC protein externalization results by Western blotting and zymography. Equal amounts of CM from the Tgfbr2FSPKO and Tgfbr2flox/flox cell lines were used for the analyses. (A) The proteins that were presented at higher levels in the Tgfbr2FSPKO cell CM. (B) The proteins that were presented at higher levels in the Tgfbr2flox/flox cell CM. (C) The proteins that were presented at equal levels in the Tgfbr2FSPKO and Tgfbr2flox/flox cell CM.
