Skip to main content
. Author manuscript; available in PMC: 2014 Sep 6.
Published in final edited form as: Proteomics. 2010 Jul;10(13):2458–2470. doi: 10.1002/pmic.200900701

Figure 5.

Figure 5

Validation of CXCL10 differential externalization and its effects on PyVmT mammary carcinoma cell proliferation and migration. (A) The “light” and “heavy” peptide ion intensities were used to calculate their corresponding differential protein level ratio. (B) The MS/MS spectra correlate with the sequences of [CNCIHIDDGPVR] confirming the identification of CXCL10. (C) The result from the CXCL10 ELISA assays of the CM from the two cell lines. (D) Escalating dosage of CXCL10 is shown to directly stimulate PyVmT cell proliferation using WST-1 cell proliferation assay (*p<0.001, **p<0.0001). (E) Escalating dosage of CXCL10 is shown to directly stimulate PyVmT cell migration using wound closure assay (*p<0.01, **p<0.001). The experiments were performed three times, with consistent results. Error bar: SD.