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. Author manuscript; available in PMC: 2015 Jan 15.
Published in final edited form as: Nat Commun. 2014 Jul 15;5:4384. doi: 10.1038/ncomms5384

Fig. 4. The toxicity associated with PrD overexpression correlates with a depletion of a subclass of Sup35 aggregates.

Fig. 4

a. Lysates from [PSI+] strains overexpressing the PrD as described in Fig. 3b that were grown in medium lacking either uracil (-Ura) or leucine (-Leu) were fractionated on sucrose gradients and analyzed by quantitative immunoblotting for Sup35 as described for Fig. 1c. The percentage of Sup35 in the pellet was determined relative to the total amount of Sup35 distributed across the gradient. Error bars represent SEM; n≥5; *p=0.017 by unpaired Student’s t-test.. b. Lysates from strains described in panel (a) were grown in medium lacking uracil (-Ura) or leucine (-Leu) and were analyzed by SDD-AGE and immunoblotting for Sup35. Lysates were normalized for Sup35 expression prior to loading to allow comparisons of aggregate size.