Skip to main content
. Author manuscript; available in PMC: 2015 Jan 15.
Published in final edited form as: Nat Commun. 2014 Jul 15;5:4384. doi: 10.1038/ncomms5384

Fig. 5. Sup35 aggregate size correlates inversely with aggregate activity and association with ribosomes.

Fig. 5

a. Lysates from [PSI+]Strong, [PSI+]Weak and [PSI+] ΔNatA strains, each expressing PrD and GST(UGA)DsRedNLS, were analyzed by SDD-AGE and immunoblotting for Sup35. b. Termination activity attributed to aggregates was determined in lysates isolated from the strains described in panel (a) by quantitative immunoblotting for GST. Error bars represent SEM; n=3; *p=0.0031 and **p=0.0012 by unpaired Student’s t-test. c. Lysates isolated from the strains described in panel (a) were immunocaptured as described in Fig. 1b for 18S ribosomal RNA. Error bars represent SEM; n=3; *p =0.001 and **p=0.0004 by unpaired Student’s t-test.