Complementation of intracellular growth defects of djlA mutant HOLD254 in J774 macrophages (A), in A549 epithelial cells (B), and in A. culbertsoni (C). Growth was measured over 72 h (A and B) or 48 h (C). The data points and error bars represent the mean CFU/well for triplicate samples from a typical experiment (performed at least twice) and their standard deviations. Symbols: ○, L. dumoffii wild-type strain; ▪, HOLD254; ▵, HOLD254-1 (djlA/pHRO18); □, HOLD254-2 (djlA/pHRO25).