FIG. 5.

Distribution of a lysosomal marker, BSA-gold, in phagosomes containing the wild-type strain or the djlA mutant strain. To label the lysosomal compartment, J774 cells were incubated with 15-nm BSA-gold overnight, washed, and then chased for 3 h. Cells were then infected with wild-type strain (A) or djlA mutant strain (B). At 4 h postinfection, the cells were fixed and processed for electron microscopy. Arrows in panel A indicate phagosomes containing no detectable gold; arrowheads in panel B indicate phagolysosomes containing BSA-gold; asterisks indicate, lysosomes containing BSA-gold. Bar, 0.5 μm.