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. Author manuscript; available in PMC: 2014 Sep 6.
Published in final edited form as: Methods Mol Biol. 2012;825:75–92. doi: 10.1007/978-1-61779-436-0_7

Figure 1.

Figure 1

Overview of tiling microarray protocol for Affymetrix Mouse 1.1R GeneChip. The mRNA population from input total RNA is first enriched by rRNA reduction. Isolated mRNA is then reverse transcribed to cDNA and amplified by in vitro transcription into cRNA. The cRNA population will be split into three reactions for another round of cDNA synthesis. The cDNA is subsequently fragmented and labeled, and ready for hybridization with GeneChip. Hybridization mix can be reused for up to three times. Therefore, the protocol is sufficient for nine GeneChip hybridization. The protocol takes three days to complete.