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. Author manuscript; available in PMC: 2014 Sep 6.
Published in final edited form as: Methods Mol Biol. 2012;825:75–92. doi: 10.1007/978-1-61779-436-0_7
Concentration of total RNA input
≥ 0.4 µg/µl ≤ 0.4 µg/µl and ≥ 0.24 µg/µl
Total RNA 3 µg 3 µg
RiboMinus Probe 2.4 µl 2.4 µl
Hybridizaation Buffer with Betaine 60 µl 90 µl
RNase-free Water up to 70µl up to 105 µl
Total Volume 70 µl 105 µl