| Component | Volume in 1 reaction |
|---|---|
| 5× 1st Strand Buffer | 2 µl |
| DTT, 0.1M | 1 µl |
| dNTP mix, 10 mM | 0.5 µl |
| RNase Inhibitor | 0.5 µl |
| SuperScript II | 1 µl |
| Total Volume | 5 µl |
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