| Component | Volume in 1 reaction |
|---|---|
| 5× 1st Strand Buffer | 4 µl |
| DTT, 0.1M | 2 µl |
| dNTP+dUTP, 10 mM | 1.25 µl |
| SuperScript II | 4.75 µl |
| Total Volume | 12 µl |
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