| Component | Volume in 1 reaction |
|---|---|
| MgCl2, 17.5 mM | 8 µl |
| dNTP+dUTP, 10 mM | 1 µl |
| DNA Polymerase I | 1.2 µl |
| RNase H | 0.5 µl |
| RNase-free water | 9.3 µl |
| Total Volume | 20 µl |
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