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. 2004 Jun;72(6):3187–3194. doi: 10.1128/IAI.72.6.3187-3194.2004

FIG. 7.

FIG. 7.

SEB prevents oxidant-induced actin depolymerization from F- to G-actin form. MSIE cells were treated with control vehicle (cont; 20 μl of ethanol), phalloidin (phal; an F-actin stabilizer as a positive control, 10 μg/ml, 120 min), cytochalasin D (cyto-D; a filamentous actin destabilizer yielding G-actin, 1 μg/ml, 15 min), or SEB (10 μg/ml, 24 h) (A) or control vehicle (cont; 20 μl of saline), SEB (10 μg/ml, 24 h), monochloramine (0.6 mM, 30 min), or SEB (10 μg/ml, 24 h) and then monochloramine (SEB/NH2Cl; 0.6 mM for 30 min after 24 h of treatment with SEB) (B). Cells were processed for globular (G) and filamentous (F) actin, as described in Materials and Methods. Images shown are representative of the results from three separate experiments.