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. Author manuscript; available in PMC: 2015 Oct 10.
Published in final edited form as: J Control Release. 2014 Mar 31;191:24–33. doi: 10.1016/j.jconrel.2014.03.041

Figure 1. Conjugation of ova to polymer carrier via a reducible disulfide linkage.

Figure 1

a) pH-responsive carrier transitions from micelle to unimer as a function of pH. HP-PDB was incubated in phosphate buffers ranging from pH 7.4 (physiologic) to 5.8 (endosomal) and particle size analyzed by dynamic light scattering, 1 mg/mL polymer. Data are from a single experiment run in triplicate with error bars representing the standard deviation. b) Fluorescence image of non-reducing SDS-PAGE validating protein-polymer conjugation via a reducible disulfide linkage, 2.8 μg ova-AF488/lane: native ova (Ova) (1), pH-responsive conjugate at 20:1 polymer:ova molar ratio [Ova-(HP-PDB)] (2), conjugate + 20mM TCEP (3), physical mixture of ova and polymer [Ova+(H-PDB)] (4), mixture + 20mM TCEP (5), non pH-responsive control conjugate [Ova-(HP-MMA)] (6), non pH-responsive control conjugate + 20mM TCEP (7). c) Hemolytic activity of diblock copolymer with [Ova-(HP-PDB)] and without (HP-PDB) antigen conjugation, of mixture control polymer (H-PDB), and of non pH-responsive control polymer (HP-MMA), at a polymer concentration of 40 μg/mL. Values are normalized relative to a positive control, 1% v/v Triton X-100, and data represent a single experiment conducted in triplicate ± SD.