Skip to main content
. Author manuscript; available in PMC: 2015 Oct 10.
Published in final edited form as: J Control Release. 2014 Mar 31;191:24–33. doi: 10.1016/j.jconrel.2014.03.041

Figure 2. Antigen conjugation enhances CTL activation/MHC-I presentation in vitro.

Figure 2

DC2.4 cells were stimulated with free protein, physical mixture, or conjugates (10 or 100 μg/mL ova) for 4 h and subsequently co-cultured with B3Z T-cells for 24 h. Cells were rinsed, incubated 24 h with lysis buffer containing chlorophenol red β-D-galactoside, and the absorbance of released chlorophenol red measured at 570 nm. Data represent a representative experiment performed in quadruplicate, mean ± SD. One-way ANOVA followed by Tukey’s Multiple Comparison Test was used for statistical analysis, * = p <0.05.