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. Author manuscript; available in PMC: 2015 Oct 10.
Published in final edited form as: J Control Release. 2014 Mar 31;191:24–33. doi: 10.1016/j.jconrel.2014.03.041

Figure 5. Conjugation enhances antigen-specific CD8+ IFN-γ+ T cell and antibody responses.

Figure 5

Splenocytes were isolated on day 29 from mice immunized twice, 3 weeks apart, with free ova, physical mixture, and pH-responsive conjugate. a) Cells were plated and tested for IFN-γ in vitro recall responses by incubating with CD8+ T cell epitope ova257-264 (SINFEKL). CD8+ T cells were identified by ICS based on CD8 expression, and the percentage of these cells expressing IFN-γ determined. Means ± SEM (n = 7–15) shown are pooled from three independent experiments. Representative flow cytometry dot plots of CD8+ IFN-γ+ T cells from individual mice. b) Ova-specific IgG1 (black bars) and IgG2c (white bars) antibody titres were measured on day 28 in sera from immunized mice. Data shown are pooled from four independent experiments and represent the mean of the reciprocal dilution ± SEM (n = 7–15). One-way ANOVA followed by Tukey’s Multiple Comparison Test was used for all statistical analyses. *p < 0.05.