FIG. 2.

Production of TbpA and TbpB in the different meningococcal TbpB-, TbpA-, or Tbp-defective M982 mutants under iron-rich (+) or iron-poor (−) growth conditions, which correspond to culture media without and with EDDHA, respectively. The detection after Western blot analysis was performed with a rabbit anti-Tbp complex purified from strain M982. The analysis of purified Tf receptor (RTf) is shown as a reference in the first lane. Each lane has been charged with the same number of cells, and data can be regarded to be semiquantitative.