FIG. 3.

HRP-hTf binding of TbpB-, TbpA-, or Tbp-defective M982 and B16B6 isogenic mutants (indicated at left) by dot blot analysis with whole cells. Serial bacterial dilutions of cells were dotted onto nitrocellulose as described in Materials and Methods. Blots were treated with HRP-conjugated hTf (1μg/ml), partially saturated with iron.