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Growth phenotype of the wild-type strain M982 and its TbpB- or TbpA-defective isogenic mutants with a mixture of apo- and holo-hTf used as the sole iron source. Growth in the presence of 200 μM EDDHA supplemented with a mixture of 2 μM holo-hTf and 8 μM apo-hTf. (▪), wild-type M982; (▴), TbpB− TbpA+ mutant PMC205; (♦), TbpB+ TbpA− mutant PMC206; (•), control (growth of M982 in the presence of EDDHA only).
