TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Genotype and phenotype | Source or reference |
|---|---|---|
| N. meningitidis strains | ||
| B16B6 | tbpB-isotype I wild type | C. Frasch |
| N16T2K | B16B6 tbpB::aphA-3 (Kmr) | 12 |
| N16T1E | B16B6 tbpA::erm (Ermr) | 12 |
| N16T12EK | B16B6 tbpB::aphA-3 tbpA::erm(Kmr Ermr) | 12 |
| M982 | tbpB-isotype II wild type | C. Frasch |
| PMC202 | M982 tbpB::ermAM inserted in orientation 1 (Ermr) | This study |
| PMC203 | M982 tbpB::ermAM inserted in orientation 2 (Ermr) | This study |
| PMC205 | M982 ΔtbpB | This study |
| PMC206 | M982 tbpA::ermAM (Ermr) | This study |
| PMC207 | M982 ΔtbpB tbpA::ermAM (Ermr) | This study |
| E. coli strain XL-1 Blue Kanr | Stratagene | |
| Plasmids | ||
| pTG1265 | Low-copy-number plasmid | 16 |
| pTG3720 | pTG1265 with XbaI fragment including tbpB and tbpA genes | 16 |
| pMGC10 | Source for ermAM (Ermr) cassette | X. Nassif |
| pIM200 | pTG1265 with tbpB 3′ region | This study |
| pIM201 | pIM200 with tbpB 5′ region | This study |
| pIM202 | pIM201 with ermAM in orientation 1 | This study |
| pIM203 | pIM201 with ermAM in orientation 2 | This study |
| pIM204 | pUC19 with tbpA 5′ region | This study |
| pIM205 | pIM204 with tbpA 3′ region | This study |
| pM1201 | pIM200 with tbpB 5′ region before its ATG | This study |
| pM1206 | pIM205 with ermAM in orientation 1 | This study |