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. 2004 Jun;72(6):3161–3170. doi: 10.1128/IAI.72.6.3161-3170.2004

FIG. 3.

FIG. 3.

Western blot of M. leprae purified rCFP-10 (lanes 1, 3, 5, 7, and 9) and M. tuberculosis purified rCFP-10 (lanes 2, 4, 6, 8, and 10) with MAbs and polyclonal antibodies. Lanes 1 and 2 contained equivalent amounts of the proteins electrophoresed on a 15% polyacrylamide gel and silver stained; lanes 3 and 4 were probed with rabbit polyclonal antiserum against M. leprae CFP-10 (rabbit αMl CFP-10) (1:10,000 dilution); lanes 5 and 6 were probed with mouse polyclonal antiserum against M. leprae CFP-10 (mouse αMl CFP-10) (1:2,500 dilution); lanes 7 and 8 were probed with mouse MAb 3A10.2C8 specific for peptides p5 and p6 (1:20 dilution of culture supernatant); and lanes 9 and 10 were probed with the mouse polyclonal antiserum against M. tuberculosis CFP-10 (mouse αMtb CFP-10) (1:2,500 dilution).