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. 2004 Jun;72(6):3161–3170. doi: 10.1128/IAI.72.6.3161-3170.2004

FIG. 4.

FIG. 4.

Western blot of subcellular fractions of M. leprae to detect native CFP-10. Lanes 1 and 6, M. leprae cytosol; lane 2, M. leprae membrane; lane 3, M. leprae cell wall; lane 4, M. tuberculosis culture filtrate protein; lane 5, M. tuberculosis cytosol. Approximately 30 μg of protein was electrophoresed in lanes 1 to 5, and 1 μg of protein was electrophoresed in lane 6. Lanes 1 to 5 were probed with purified IgG from rabbit polyclonal anti-M. leprae CFP-10, while lane 6 was probed with MAb CS-01 specific for the 10-kDa GroEs protein. mw, molecular weight (in thousands).