FIG. 5.

Comparison of M. tuberculosis CFP-10-specific T-cell hybridoma responses to the four peptide epitopes recognized. T-cell hybridomas were cultured with the antigen-presenting B-cell line A20 pulsed with serial twofold dilutions of peptide (4 μg to 8 ng per well) in a dose-response experiment. The hybridomas examined were 7F12 and 3A2 (peptide p1 specific), 5B12 and 6D5 (p4 specific), 1G10 and 5H9 (p9 specific), and 6A3 and 9H1 (p15 and p16 specific). The IL-2 secretion in response to antigen stimulation was measured in 24-h culture supernatant by a capture ELISA. O.D. 450 nm, optical density at 450 nm.