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AOAH is synthesized in the renal cortex. (A) AOAH activity in lysates of renal cortex, medulla, and washed bladder. Each bar shows the mean and standard error of three or more measurements. The mean activity in seven bladder samples was 2.5% of the activity in total kidney lysates. (B) Real-time PCR analysis of AOAH and GAPDH mRNA in renal cortex, medulla, and bladder. This experiment was performed in duplicate and repeated twice, using tissues from different mice, with similar results.
