FIG. 5.

AOAH activity in mouse urine. (A) LPS deacylation by urine from AOAH +/+ and −/− mice. Urine (10 μl) was added to 490 μl of AOAH reaction mixture containing 1 μg of [³H/¹⁴C]LPS and incubated at 37°C for 18 h before the addition of ethanol and further steps as described in Materials and Methods. (B) LPS deacylation by murine urine. Fresh urine (10 μl) was incubated with [³H/¹⁴C]LPS (0.5 μg) at 4 and 37°C for 18 h. AOAH reaction mixture was then added to provide protein for coprecipitation of intact LPS, followed by ethanol. The remaining steps are described in Materials and Methods. Each datum point represents a different mouse.