FIG. 6.

Mature AOAH is found in urine. Equal volumes of wild-type and AOAH-null urine were immunoprecipitated with an anti-murine AOAH monoclonal antibody and assayed by Western blot as described in Materials and Methods. Lysates of BHK cells transfected with AOAH were used as the positive control. The results are representative of three experiments with similar results. Note in the upper panel that the BHK-AOAH cell lysate contains both pro-AOAH (open arrow) and mature AOAH (solid arrow). Wild-type urine only has mature AOAH. After treatment with DTT (lower panel), mature AOAH migrates with an apparent M_r of 50 kDa (solid arrow). The band at an apparent M_r of 55 kDa in the lower panel is the heavy chain of the murine monoclonal antibody used for immunoprecipitation.