Table 2.
Morphokinetic parameters and Results of Two Way Analysis of Variance (ANOVA) for patients, ploidy and interaction
| Morphokinetic Parameters a | PB extr | PN abutt | Furrow | 2 cells | Len div | Cc2 | 3 cell | 4 cell | 3cell-4cell | 5 cell | 8 cell | Starts comp | Ends comp | Durat comp | Starts cav | blast |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mean ± stdbdev | −20 ± 2.8 | −12 ± 2.9 | 2.2 ± 0.39 | 2.8 ± 1.0 | 0.7 ± 0.98 | 11 ± 2.4 | 14 ± 2.1 | 15 ± 1.8 | 1.0 ± 2.0 | 27 ± 4.5 | 33 ± 6.1 | 50 ± 12 | 59 ± 11 | 13 ± 7.6 | 72 ± 9.1 | 84 ± 10.0 |
| F (pts) c | 2.58 | 2.81 | 2.75 | 2.82 | 0.65 | 3.39 | 3.45 | 3.45 | 0.54 | 3.44 | 2.77 | 3.48 | 3.35 | 2.60 | 3.73 | 3.84 |
| d.f | 4/11 | 4/12 | 17/52 | 18/68 | 17/52 | 18/67 | 18/67 | 18/67 | 18/67 | 18/67 | 18/56 | 23/91 | 18/65 | 19/65 | 23/89 | 22/89 |
| sig | NS | NS | Sig. | Sig | NS | Sig. | Sig. | Sig. | NS | Sig. | Sig. | Sig. | Sig. | Sig. | Sig. | Sig. |
| F (ploidy) d | .12 | .07 | .08 | .22 | .71 | .01 | .03 | .02 | .00 | .03 | .01 | .07 | .04 | 2.70 | .01 | .03 |
| d.f. | 1/11 | 1/12 | 1/52 | 1/68 | 1/52 | 1/67 | 1/67 | 1/67 | 1/67 | 1/67 | 1/56 | 1/91 | 1/65 | 1/65 | 1/91 | 1/89 |
| sig | NS | NS | NS | NS | NS | NS | NS | NS | NS | NS | NS | NS | NS | NS | NS | NS |
| F (intrct) e | 0.27 | 0.01 | 0.11 | 0.08 | .00 | 0.17 | .16 | .13 | .01 | .15 | .17 | .24 | .14 | .27 | .22 | .23 |
| d.f. | 4/11 | 4/12 | 17/52 | 18/68 | 17/52 | 18/67 | 18/67 | 18/67 | 18/67 | 18/67 | 18/56 | 23/91 | 18/65 | 19/65 | 23/91 | 22/89 |
| sig | NS | NS | NS | NS | NS | NS | NS | NS | NS | NS | NS | NS | NS | NS | NS | NS |
a Time of second polar body extrusion (PB extr). Time of initial juxtaposition and centralization of PN’s (PN abutt). Time zygote undergoes first mitosis and beginnings of zygotic groove is seen (Furrow). Time of first embryo division to two distinct daughter cells (2 cells). Time interval between Furrow and 2 cells (Len div). Time interval between 2 cells 3 and cell (Cc2). Time of division into three distinct daughter cells (3 cell). Time of division into four distinct daughter cells (4 cell). Time interval between 3 cell to 4 cell (3cell-4cell). Time of division into five distinct daughter cells (5 cell). Time of division into eight distinct daughter cells (8 cell). Time when cells within embryo begin to adhere and lose an individual appearance (Starts comp) [5]. Time when embryo appears as indistinguishable mass of cells where cell boundaries are unclear (Ends Comp). Time interval between Starts comp and Ends comp (Dur comp). Earliest stage of a blastocyst with a small cavity visible (Starts cav) [5]. Time of formation of full blastocyst; when blastocoele filled embryo with <10%increase in diameter (blast) [5]
b mean ± standard deviation for morphokinetic parameters (presented in hours from syngamy). Negative values indicate morphokinetic parameters that preceded syngamy
c F value for patients (mean squared deviation among patients/mean squared deviation within ploidy groups)
d F value for ploidy (mean squared deviation among ploidy types (aneuploid and euploid)/mean squared deviation within ploidy groups)
e F value for interaction (mean squared deviation for interaction between ploidy and patients/mean squared deviation within ploidy groups)