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. 2004 Jun;72(6):3204–3217. doi: 10.1128/IAI.72.6.3204-3217.2004

FIG. 2.

FIG. 2.

Virulent RCI of F. tularensis colocalizes extensively with LAMP CD63 at 4 h but very little at 16 h after uptake by human THP-1 cells. THP-1 cells were infected with the RCI of F. tularensis, fixed 4 or 16 h after infection, stained for CD63 (Oregon Green), F. tularensis (Texas Red), and DNA (DAPI), and imaged by laser scanning confocal and two-photon fluorescence microscopy. After 4 h (top row), the majority of F. tularensis bacteria resided in compartments with intense staining around the rim for CD63 (arrows). After 16 h (bottom row), the bacteria had multiplied extensively and no longer colocalized with CD63. This experiment was performed twice, with similar results.