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. 2014 Sep 4;12:245. doi: 10.1186/s12967-014-0245-7

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© Fisher et al.; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Enhanced immunological memory following CD4 T cell depletion. Polychromatic flow cytometry was used to determine the proportion of CD8+ CD44+ CD62L- effector memory (T_EM) T cells in spleen (Spln) and lymph nodes (LN) of the tumor rechallenged mice from Figure 2B. Significantly more T_EM were observed in LN and Spln from all CD4 depleted groups compared to control mice. * = p <0.05, ***p < 0.001, ****p < 0.0001.