FIG. 2.

TLR4 and CD14 surface expression on excreted urinary tract epithelial cells. Urinary epithelial cells were harvested from healthy individuals, and the expression of TLR4 and CD14 was analyzed by flow cytometry. The shed cells stained strongly for TLR4 (A), but CD14 was not detected (D). Epithelial cells were identified by using the cytokeratin antibody MNF116 (not shown). The specificity of the anti-TLR4 antibody was confirmed by using HEK 293 cells that did not express tlr4 (B) and HEK 293 cells transfected with tlr4 (C). CD14-expressing blood monocytes were used as positive controls for anti-CD14 antibodies (E).