TABLE 2.

Epithelial cytokine response to in vitro challenge with E. coli

Strain	Cytokine	h	Cytokine secretion (pg/ml)a
			Challenge	Controlb	P value
Hu734	IL-6	0	<50	<50	NSc
		2	612 ± 73	185 ± 13	<0.001
		6	2,692 ± 395	337 ± 24	<0.001
	IL-8	0	<50	<50	NS
		2	934 ± 240	132 ± 7	<0.001
		6	11,460 ± 1,238	223 ± 10	<0.001
pap	IL-6	0	<50	<50	NS
		2	1,060 ± 233	524 ± 194	NS
		6	4,342 ± 152	998 ± 136	<0.001
	IL-8	0	119 ± 36	53 ± 7	NS
		2	433 ± 132	235 ± 18	0.057
		6	14,352 ± 647	2,059 ± 284	<0.001
fim	IL-6	0	<50	<50	NS
		2	1,451 ± 307	494 ± 81	0.029
		6	4,284 ± 190	994 ± 107	<0.001
	IL-8	0	100 ± 4	51 ± 13	0.03
		2	1,538 ± 967	346 ± 37	NS
		6	16,896 ± 744	2,673 ± 743	<0.001

^aCytokines in supernatants obtained at various times after in vitro challenge of the biopsies were quantified. Values are means ± standard errors of the means.

^bControl biopsies were incubated in RPMI. Controls for the pap⁺ and fim⁺ strains were pap- and fim-negative strains, respectively.

^cNS, not significant.